X-ray diffraction
2.09Å resolution

Structure of Bacillus subtilis Nitric Oxide Synthase in complex with 6,6'-((5-(aminomethyl)-1,3-phenylene)bis(ethane-2,1-diyl))bis(4- methylpyridin-2-amine)

Source organism: Bacillus subtilis
Entry authors: Holden JK, Poulos TL

Function and Biology Details

Reaction catalysed:
(1a) 2 L-arginine + 2 reduced flavodoxin + 2 O(2) = 2 N(omega)-hydroxy-L-arginine + 2 oxidized flavodoxin + 2 H(2)O
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Nitric oxide synthase oxygenase Chain: A
Molecule details ›
Chain: A
Length: 363 amino acids
Theoretical weight: 41.79 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
  • Canonical: O34453 (Residues: 1-363; Coverage: 100%)
Gene names: BSU07630, nos, yflM
Sequence domains: Nitric oxide synthase, oxygenase domain
Structure domains:

Ligands and Environments

Cofactor: Ligand HEM 1 x HEM
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21212
Unit cell:
a: 80.51Å b: 94.94Å c: 63.49Å
α: 90° β: 90° γ: 90°
R R work R free
0.17 0.167 0.22
Expression system: Escherichia coli BL21(DE3)