X-ray diffraction
2.1Å resolution

Crystal structure of the Bombyx mori Vasa helicase (E339Q) in complex with RNA,ADP and Pi


Function and Biology Details

Reaction catalysed:
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNA helicase Chain: A
Molecule details ›
Chain: A
Length: 434 amino acids
Theoretical weight: 48.13 KDa
Source organism: Bombyx mori
Expression system: Escherichia coli BL21(DE3)
  • Canonical: O01378 (Residues: 135-564; Coverage: 72%)
Gene names: 692765, BmVLG
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases
5'-R(*UP*GP*AP*CP*AP*UP)-3' Chain: D
Molecule details ›
Chain: D
Length: 6 nucleotides
Theoretical weight: 1.88 KDa
Source organism: Bombyx mori
Expression system: Not provided

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 52.96Å b: 77.81Å c: 107.3Å
α: 90° β: 90° γ: 90°
R R work R free
0.229 0.225 0.277
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided