4cwc

X-ray diffraction
2.9Å resolution

Structure of Rolling Circle Replication Initiator Protein (RepDE) from Staphylococcus aureus

Released:
Source organism: Staphylococcus aureus
Entry authors: Carr SB, Phillips SEV, Thomas CD

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Replication initiation protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 281 amino acids
Theoretical weight: 34.28 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P12053 (Residues: 230-314; Coverage: 27%)
  • Canonical: P03065 (Residues: 32-226; Coverage: 63%)
Gene names: repD, repE
Sequence domains: Replication initiation factor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2
Unit cell:
a: 240.3Å b: 56.536Å c: 62.357Å
α: 90° β: 102.22° γ: 90°
R-values:
R R work R free
0.219 0.216 0.259
Expression system: Escherichia coli