› 4cng

X-ray diffraction
1.1Å resolution

Crystal structure of Sulfolobus acidocaldarius TrmJ in complex with S-adenosyl-L-Homocysteine

Released:
DOI: 10.2210/pdb4cng/pdb
PDB entry 4cng coloured by chain, front view.
PDB entry 4cng coloured by chain, side view.
PDB entry 4cng coloured by chain, top view.
Source organism: Sulfolobus acidocaldarius
Primary publication:
Characterization of two homologous 2'-O-methyltransferases showing different specificities for their tRNA substrates.
Somme J, Van Laer B, Roovers M, Steyaert J, Versées W, Droogmans L
RNA 20 1257-71 (2014)
PMID: 24951554

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA (cytidine-2'-O-)-methyltransferase TrmJ Chains: A, B
Molecule details ›
Chains: A, B
Length: 255 amino acids
Theoretical weight: 29.08 KDa
Source organism: Sulfolobus acidocaldarius
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4JB16 (Residues: 1-235; Coverage: 100%)
Gene names: Saci_0621, trmJ
Sequence domains: SpoU rRNA Methylase family
Structure domains: Alpha/beta knot

Ligands and Environments

2 bound ligands:
Ligand SAH 3 x SAH
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Unit cell:
a: 45.803Å b: 54.097Å c: 130.637Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.162 0.185
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

RNA 2'-O-Methylation (Nm) Modification in Human Diseases.
Dimitrova et al. (2019)
18 more