X-ray diffraction
2.05Å resolution

Structure of 55 kDa N-terminal domain of E. coli DNA gyrase A subunit with simocyclinone D8 bound


Function and Biology Details

Reaction catalysed:
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
DNA gyrase subunit A Chains: A, B
Molecule details ›
Chains: A, B
Length: 493 amino acids
Theoretical weight: 55.38 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
  • Canonical: A0A0H3JH39 (Residues: 30-522; Coverage: 56%)
Gene names: ECs_3114, gyrA
Sequence domains: DNA gyrase/topoisomerase IV, subunit A
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2
Unit cell:
a: 160.82Å b: 96.05Å c: 112.35Å
α: 90° β: 132.72° γ: 90°
R R work R free
0.197 0.196 0.225
Expression system: Escherichia coli