Electron Microscopy
9.2Å resolution

Pseudo-atomic model of microtubule-bound human kinesin-5 motor domain in presence of adp.alfx (NECK-LINKER IN ITS DISCONNECTED CONFORMATION, based on cryo-electron microscopy experiment


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1D chain Chain: A
Molecule details ›
Chain: A
Length: 452 amino acids
Theoretical weight: 50.24 KDa
Source organism: Bos taurus
  • Canonical: Q2HJ86 (Residues: 1-452; Coverage: 100%)
Gene name: TUBA1D
Sequence domains:
Tubulin beta-2B chain Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 49.91 KDa
Source organism: Bos taurus
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Sequence domains:
Kinesin-like protein KIF11 Chain: C
Molecule details ›
Chain: C
Length: 373 amino acids
Theoretical weight: 41.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
  • Canonical: P52732 (Residues: 1-367; Coverage: 35%)
Gene names: EG5, KIF11, KNSL1, TRIP5
Sequence domains: Kinesin motor domain

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 9.2Å
Relevant EMDB volumes: EMD-2533
Expression system: Escherichia coli BL21