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X-ray diffraction
1.3Å resolution

Crystal structure of the N-terminal domain of the PA subunit of Dhori virus polymerase

Released:
DOI: 10.2210/pdb4cgs/pdb
PDB 4cgs coloured by chain and viewed from the front.
PDB 4cgs coloured by chain and viewed from the side.
PDB 4cgs coloured by chain and viewed from the top.
Source organism: Dhori thogotovirus
Primary publication:
Comparative structural and functional analysis of orthomyxovirus polymerase cap-snatching domains.
Guilligay D, Kadlec J, Crépin T, Lunardi T, Bouvier D, Kochs G, Ruigrok RW, Cusack S
OpenAccess logo PLoS ONE 9 e84973 (2014)
PMID: 24454773

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase subunit PA Chains: A, B
Molecule details ›
Chains: A, B
Length: 173 amino acids
Theoretical weight: 20.27 KDa
Source organism: Dhori thogotovirus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D6PT85 (Residues: 1-171; Coverage: 27%)
Gene name: PA
Structure domains: Influenza RNA-dependent RNA polymerase subunit PA, endonuclease domain

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
1 modified residue:
Ligand CME 2 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 43.91Å b: 87.45Å c: 56.13Å
α: 90° β: 112.98° γ: 90°
R-values:
R R work R free
0.175 0.173 0.22
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Modern Approaches for Identification of Modified Nucleotides in RNA.
Filippova et al. (2017)
3 more