4ce0

X-ray diffraction
2Å resolution

Crystal Structure of SAH-bound Spinosyn Rhamnosyl 4'-O- Methyltransferase SpnH from Saccharopolyspora spinosa

Released:
Source organism: Saccharopolyspora spinosa
Entry authors: Lin Y-C, Huang S-P, Huang B-L, Chen Y-H, Chen Y-J, Chiu H-T

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
O-methyltransferase/macrocin O-methyltransferase/8-demethyl-8-(2, 3-dimethoxy-alpha-L-rhamnosyl)tetracenomycin-C 4'-O-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 29.15 KDa
Source organism: Saccharopolyspora spinosa
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9ALM9 (Residues: 1-250; Coverage: 100%)
Gene names: A8926_6436, spnH
Sequence domains: Macrocin-O-methyltransferase (TylF)

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: C2
Unit cell:
a: 52.21Å b: 72.798Å c: 61.793Å
α: 90° β: 106.05° γ: 90°
R-values:
R R work R free
0.176 0.17 0.227
Expression system: Escherichia coli BL21