4cd8

X-ray diffraction
1.47Å resolution

The structure of GH113 beta-mannanase AaManA from Alicyclobacillus acidocaldarius in complex with ManMIm

Released:

Function and Biology Details

Reaction catalysed:
Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endo-beta-1,4-mannanase Chain: A
Molecule details ›
Chain: A
Length: 320 amino acids
Theoretical weight: 36.34 KDa
Source organism: Alicyclobacillus acidocaldarius
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A5H1I6 (Residues: 1-320; Coverage: 100%)
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 43.82Å b: 75.89Å c: 91.48Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.17 0.215
Expression system: Escherichia coli BL21