4ccg

X-ray diffraction
2.4Å resolution

Structure of an E2-E3 complex

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin-conjugating enzyme E2 T Chains: A, B
Molecule details ›
Chains: A, B
Length: 212 amino acids
Theoretical weight: 24.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NPD8 (Residues: 1-197; Coverage: 100%)
Gene names: HSPC150, PIG50, UBE2T
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme
E3 ubiquitin-protein ligase FANCL Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 88 amino acids
Theoretical weight: 10.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NW38 (Residues: 288-375; Coverage: 24%)
Gene names: FANCL, PHF9
Sequence domains: FANCL C-terminal domain
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P43212
Unit cell:
a: 109.224Å b: 109.224Å c: 117.728Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.248
Expression system: Escherichia coli BL21(DE3)