› 4cbb

X-ray diffraction
1.8Å resolution

APO FORM OF BETAINE ALDEHYDE DEHYDROGENASE FROM Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb4cbb/pdb
PDB entry 4cbb coloured by chain, front view.
PDB entry 4cbb coloured by chain, side view.
PDB entry 4cbb coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Entry authors: Gonzalez-Segura L, Diaz-Sanchez AG, Munoz-Clares RA

Function and Biology Details

Reaction catalysed: 
Betaine aldehyde + NAD(+) + H(2)O = betaine + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190927 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD/NADP-dependent betaine aldehyde dehydrogenase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 489 amino acids
Theoretical weight: 53.26 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HTJ1 (Residues: 2-490; Coverage: 100%)
Gene names: PA5373, betB
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand DTT 8 x DTT
Ligand EDO 10 x EDO
Ligand PE4 8 x PE4
Ligand GOL 22 x GOL
Ligand K 24 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 332.32Å b: 131.339Å c: 100.815Å
α: 90° β: 95.02° γ: 90°
R-values:
R R work R free
0.149 0.147 0.183
Expression system: Escherichia coli BL21(DE3)

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