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Architecture of the Lsm1-7-Pat1 complex: a conserved assembly in eukaryotic mRNA turnover.

Sharif H, Conti E
Cell Rep 5 283-91 (2013)
Cited: 47 times
EuropePMC logo PMID: 24139796

Abstract

The decay of mRNAs is a key step in eukaryotic gene expression. The cytoplasmic Lsm1-7-Pat1 complex is a conserved component of the 5'-to-3' mRNA decay pathway, linking deadenylation to decapping. Lsm1-7 is similar to the nuclear Sm complexes that bind oligo-uridine tracts in snRNAs. The 2.3 Å resolution structure of S. cerevisiae Lsm1-7 shows the presence of a heptameric ring with Lsm1-2-3-6-5-7-4 topology. A distinct structural feature of the cytoplasmic Lsm ring is the C-terminal extension of Lsm1, which plugs the exit site of the central channel and approaches the RNA binding pockets. The 3.7 Å resolution structure of Lsm1-7 bound to the C-terminal domain of Pat1 reveals that Pat1 recognition is not mediated by the distinguishing cytoplasmic subunit, Lsm1, but by Lsm2 and Lsm3. These results show how the auxiliary domains and the canonical Sm folds of the Lsm1-7 complex are organized in order to mediate and modulate macromolecular interactions.

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Articles - 4c92 mentioned but not cited (3)

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  6. A unique surface on Pat1 C-terminal domain directly interacts with Dcp2 decapping enzyme and Xrn1 5'-3' mRNA exonuclease in yeast. Charenton C, Gaudon-Plesse C, Fourati Z, Taverniti V, Back R, Kolesnikova O, Séraphin B, Graille M. Proc Natl Acad Sci U S A 114 E9493-E9501 (2017)
  7. Pat1 contributes to the RNA binding activity of the Lsm1-7-Pat1 complex. Chowdhury A, Kalurupalle S, Tharun S. RNA 20 1465-1475 (2014)
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  9. The Lsm1-7-Pat1 complex promotes viral RNA translation and replication by differential mechanisms. Jungfleisch J, Chowdhury A, Alves-Rodrigues I, Tharun S, Díez J. RNA 21 1469-1479 (2015)
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  16. The C-terminal domain from S. cerevisiae Pat1 displays two conserved regions involved in decapping factor recruitment. Fourati Z, Kolesnikova O, Back R, Keller J, Charenton C, Taverniti V, Plesse CG, Lazar N, Durand D, van Tilbeurgh H, Séraphin B, Graille M. PLoS One 9 e96828 (2014)
  17. The sole LSm complex in Cyanidioschyzon merolae associates with pre-mRNA splicing and mRNA degradation factors. Reimer KA, Stark MR, Aguilar LC, Stark SR, Burke RD, Moore J, Fahlman RP, Yip CK, Kuroiwa H, Oeffinger M, Rader SD. RNA 23 952-967 (2017)
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  20. Defining essential elements and genetic interactions of the yeast Lsm2-8 ring and demonstration that essentiality of Lsm2-8 is bypassed via overexpression of U6 snRNA or the U6 snRNP subunit Prp24. Roth AJ, Shuman S, Schwer B. RNA 24 853-864 (2018)
  21. The cytoplasmic mRNA degradation factor Pat1 is required for rRNA processing. Muppavarapu M, Huch S, Nissan T. RNA Biol 13 455-465 (2016)
  22. Variants in LSM7 impair LSM complexes assembly, neurodevelopment in zebrafish and may be associated with an ultra-rare neurological disease. Derksen A, Shih HY, Forget D, Darbelli L, Tran LT, Poitras C, Guerrero K, Tharun S, Alkuraya FS, Kurdi WI, Nguyen CE, Laberge AM, Si Y, Gauthier MS, Bonkowsky JL, Coulombe B, Bernard G. HGG Adv 2 100034 (2021)
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  26. LSM1-mediated Major Satellite RNA decay is required for nonequilibrium histone H3.3 incorporation into parental pronuclei. Zhu J, Chen K, Sun YH, Ye W, Liu J, Zhang D, Su N, Wu L, Kou X, Zhao Y, Wang H, Gao S, Kang L. Nat Commun 14 957 (2023)
  27. The expression pattern of OsDim1 in rice and its proposed function. Doku HA, Gan SX, Zhu Q, Nadir S, Li W, Li MT, Zhou L, Li CY, Kang SG, Park EH, Chen LJ, Lee DS. Sci Rep 9 18492 (2019)


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