X-ray diffraction
2.7Å resolution

Phenylacetone monooxygenase: oxidised R337K mutant in complex with APADP

Source organism: Thermobifida fusca
Entry authors: Martinoli C, Fraaije MW, Mattevi A

Function and Biology Details

Reaction catalysed:
Phenylacetone + NADPH + O(2) = benzyl acetate + NADP(+) + H(2)O

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Phenylacetone monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 542 amino acids
Theoretical weight: 61.16 KDa
Source organism: Thermobifida fusca
Expression system: Escherichia coli K-12
  • Canonical: Q47PU3 (Residues: 1-542; Coverage: 100%)
Gene names: Tfu_1490, pamO
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments

Cofactor: Ligand FAD 1 x FAD

Cofactor: Ligand N01 1 x N01
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14
Spacegroup: P3221
Unit cell:
a: 107.36Å b: 107.36Å c: 106.79Å
α: 90° β: 90° γ: 120°
R R work R free
0.196 0.193 0.257
Expression system: Escherichia coli K-12