4c45

X-ray diffraction
1.45Å resolution

Crystal structure of human pterin-4-alpha-carbinolamine dehydratase 2 (PCBD2)

Released:
Source organism: Homo sapiens
Entry authors: Kopec J, Kiyani W, Vollmar M, Shrestha L, Canning P, von Delft F, Burgess-Brown N, Bountra C, Arrowsmith CH, Edwards A, Yue WW

Function and Biology Details

Reaction catalysed:
4a-hydroxytetrahydrobiopterin = 6,7-dihydrobiopterin + H(2)O
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pterin-4-alpha-carbinolamine dehydratase 2 Chain: A
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 11.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H0N5 (Residues: 28-130; Coverage: 79%)
Gene names: DCOH2, DCOHM, PCBD2
Sequence domains: Pterin 4 alpha carbinolamine dehydratase
Structure domains: Transcriptional coactivator/pterin dehydratase

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C222
Unit cell:
a: 51.547Å b: 94.044Å c: 48.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.144 0.167
Expression system: Escherichia coli BL21(DE3)