X-ray diffraction
1.64Å resolution

Human N-myristoyltransferase (NMT1) with Myristoyl-CoA co-factor

Source organism: Homo sapiens
Entry authors: Thinon E, Serwa RA, Brannigan JA, Brassat U, Wright MH, Heal WP, Wilkinson AJ, Mann DJ, Tate EW

Function and Biology Details

Reaction catalysed:
Tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein]
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Glycylpeptide N-tetradecanoyltransferase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 410 amino acids
Theoretical weight: 47.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P30419 (Residues: 109-496; Coverage: 78%)
Gene names: NMT, NMT1
Sequence domains:
Structure domains: Aminopeptidase

Ligands and Environments

Cofactor: Ligand MYA 2 x MYA
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21212
Unit cell:
a: 78.74Å b: 178.48Å c: 58.55Å
α: 90° β: 90° γ: 90°
R R work R free
0.173 0.17 0.222
Expression system: Escherichia coli BL21(DE3)