X-ray diffraction
1.47Å resolution

Structure of the Mycobacterium tuberculosis APS kinase CysC in complex with ADP


Function and Biology Details

Reactions catalysed:
ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate
ATP + sulfate = diphosphate + adenylyl sulfate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Bifunctional enzyme CysN/CysC; Multifunctional fusion protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 174 amino acids
Theoretical weight: 19.14 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P9WNM5 (Residues: 440-612; Coverage: 28%)
  • Canonical: A5U1Y4 (Residues: 440-612; Coverage: 28%)
Gene names: MRA_1294, MTCY373.05, Rv1286, cysC, cysN, cysNC
Sequence domains: Adenylylsulphate kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 43.63Å b: 66.33Å c: 61.76Å
α: 90° β: 103.59° γ: 90°
R R work R free
0.16 0.158 0.182
Expression system: Escherichia coli BL21(DE3)