› 4bya

Solution NMR

Calmodulin, C-terminal domain, M144H mutant

Released:
DOI: 10.2210/pdb4bya/pdb
PDB entry 4bya coloured by chain, ensemble of 20 models, front view.
PDB entry 4bya coloured by chain, ensemble of 20 models, side view.
PDB entry 4bya coloured by chain, ensemble of 20 models, top view.
Source organism: Gallus gallus
Primary publication:
New Tricks for Old Proteins: Single Mutations in a Nonenzymatic Protein Give Rise to Various Enzymatic Activities.
Moroz YS, Dunston TT, Makhlynets OV, Moroz OV, Wu Y, Yoon JH, Olsen AB, McLaughlin JM, Mack KL, Gosavi PM, van Nuland NA, Korendovych IV
J Am Chem Soc 137 14905-11 (2015)
PMID: 26555770

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158569 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calmodulin Chain: A
Molecule details ›
Chain: A
Length: 75 amino acids
Theoretical weight: 8.62 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62149 (Residues: 77-145; Coverage: 46%)
Gene names: CALM, CAM, RCJMB04_24e7
Sequence domains: EF-hand domain pair

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 91%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Conformational dynamics and enzyme evolution.
Petrović et al. (2018)
9 more

1 mention without citation

A Designed Enzyme Promotes Selective Post-translational Acylation.
Gosavi et al. (2018)