4bss

X-ray diffraction
3.2Å resolution

Structure of the ectodomain of LGR5 in complex with R-spondin-1 (Fu1Fu2) in P21 crystal form

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Leucine-rich repeat-containing G-protein coupled receptor 5 Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 539 amino acids
Theoretical weight: 60.37 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O75473 (Residues: 22-543; Coverage: 59%)
Gene names: GPR49, GPR67, LGR5
Sequence domains:
Structure domains: Ribonuclease Inhibitor
R-spondin-1 Chains: C, D, G, H
Molecule details ›
Chains: C, D, G, H
Length: 126 amino acids
Theoretical weight: 13.71 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2MKA7 (Residues: 31-146; Coverage: 48%)
Gene name: RSPO1
Structure domains: Hormone Receptor, Insulin-like Growth Factor Receptor 1; Chain A, domain 2

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 120.362Å b: 111.298Å c: 130.611Å
α: 90° β: 109.19° γ: 90°
R-values:
R R work R free
0.249 0.246 0.29
Expression system: Homo sapiens