4brb

X-ray diffraction
2.55Å resolution

Crystal structure of the integral membrane enzyme DgkA-ref, delta 7

Released:
Source organism: Escherichia coli K-12
Entry authors: Li D, Caffrey M

Function and Biology Details

Reaction catalysed:
ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diacylglycerol kinase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 130 amino acids
Theoretical weight: 14.2 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABN1 (Residues: 2-122; Coverage: 99%)
Gene names: JW4002, b4042, dgk, dgkA
Sequence domains: Prokaryotic diacylglycerol kinase
Structure domains: Helix Hairpins

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 75.5Å b: 91.57Å c: 143.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.217 0.261
Expression system: Escherichia coli