4bq8

X-ray diffraction
2.8Å resolution

Crystal structure of the RGMB-NEO1 complex form 3

Released:
Source organisms:
Entry authors: Bell CH, Healey E, van Erp S, Bishop B, Tang C, Gilbert RJC, Aricescu AR, Pasterkamp RJ, Siebold C

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Neogenin Chain: A
Molecule details ›
Chain: A
Length: 213 amino acids
Theoretical weight: 24.08 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: P97798 (Residues: 883-1083; Coverage: 14%)
Gene names: Neo1, Ngn
Sequence domains: Fibronectin type III domain
Structure domains: Immunoglobulins
RGM domain family member B Chain: B
Molecule details ›
Chain: B
Length: 122 amino acids
Theoretical weight: 13.32 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6NW40 (Residues: 50-168; Coverage: 30%)
Gene name: RGMB
RGM domain family member B Chain: C
Molecule details ›
Chain: C
Length: 251 amino acids
Theoretical weight: 27.95 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6NW40 (Residues: 169-410; Coverage: 62%)
Gene name: RGMB
Sequence domains: Repulsive guidance molecule (RGM) C-terminus
Structure domains: Mog1/PsbP, alpha/beta/alpha sandwich

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P41212
Unit cell:
a: 116.92Å b: 116.92Å c: 91.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.183 0.199
Expression system: Homo sapiens