4bq6

X-ray diffraction
2.3Å resolution

Crystal structure of the RGMB-NEO1 complex form 1

Released:
Source organisms:
Entry authors: Bell CH, Healey E, van Erp S, Bishop B, Tang C, Gilbert RJC, Aricescu AR, Pasterkamp RJ, Siebold C

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Neogenin Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 29.22 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: P97798 (Residues: 883-1133; Coverage: 17%)
Gene names: Neo1, Ngn
Sequence domains: Fibronectin type III domain
Structure domains: Immunoglobulins
Repulsive guidance molecule B Chains: C, E
Molecule details ›
Chains: C, E
Length: 122 amino acids
Theoretical weight: 13.32 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6NW40 (Residues: 50-168; Coverage: 30%)
Gene name: RGMB
Repulsive guidance molecule B Chains: D, F
Molecule details ›
Chains: D, F
Length: 251 amino acids
Theoretical weight: 27.95 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6NW40 (Residues: 169-410; Coverage: 62%)
Gene name: RGMB
Sequence domains: Repulsive guidance molecule (RGM) C-terminus
Structure domains: Mog1/PsbP, alpha/beta/alpha sandwich

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 91.32Å b: 100.39Å c: 103.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.218 0.266
Expression system: Homo sapiens