› 4bpb

X-ray diffraction
2.58Å resolution

STRUCTURAL INSIGHTS INTO RNA RECOGNITION BY RIG-I

Released:
DOI: 10.2210/pdb4bpb/pdb
PDB entry 4bpb coloured by chain, front view.
PDB entry 4bpb coloured by chain, side view.
PDB entry 4bpb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into RNA recognition by RIG-I.
Luo D, Ding SC, Vela A, Kohlway A, Lindenbach BD, Pyle AM
Cell 147 409-22 (2011)
PMID: 22000018

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132024 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Antiviral innate immune response receptor RIG-I Chain: A
Molecule details ›
Chain: A
Length: 698 amino acids
Theoretical weight: 79.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95786 (Residues: 230-671, 672-925; Coverage: 75%)
Gene names: DDX58, RIGI
Sequence domains:
Structure domains:
5'-R(*GP*CP*GP*CP*GP*CP*GP*CP*GP*CP)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 10 nucleotides
Theoretical weight: 3.21 KDa

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 47.618Å b: 76.208Å c: 219.825Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.195 0.255
Expression system: Escherichia coli

Quick links

Citations

99 review citations

Innate immune sensing and signaling of cytosolic nucleic acids.
Wu et al. (2014)
98 more

4 mentions without citation

Host DDX Helicases as Possible SARS-CoV-2 Proviral Factors: A Structural Overview of Their Hijacking Through Multiple Viral Proteins.
Squeglia et al. (2020)
3 more