4bou

X-ray diffraction
1.55Å resolution

Function and Biology Details

Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
OTU domain-containing protein 3 Chain: A
Molecule details ›
Chain: A
Length: 160 amino acids
Theoretical weight: 18.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5T2D3 (Residues: 52-209; Coverage: 40%)
Gene names: KIAA0459, OTUD3
Sequence domains: OTU-like cysteine protease
Structure domains: Cathepsin B; Chain A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P3212
Unit cell:
a: 36.196Å b: 36.196Å c: 211.051Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.175 0.229
Expression system: Escherichia coli BL21(DE3)