› 4bmn

X-ray diffraction
1.5Å resolution

Function and Biology Details

Reaction catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110982 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alclohol dehydrogenase/short-chain dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 253 amino acids
Theoretical weight: 27.11 KDa
Source organism: Ralstonia sp. DSMZ 6428
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C0IR58 (Residues: 1-249; Coverage: 100%)
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand EDO 8 x EDO
Ligand TAM 1 x TAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2
Unit cell:
a: 136.48Å b: 52.51Å c: 151.47Å
α: 90° β: 116.57° γ: 90°
R-values:
R R work R free
0.16 0.158 0.188
Expression system: Escherichia coli BL21(DE3)

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