4blv

X-ray diffraction
2Å resolution

Crystal structure of Escherichia coli 23S rRNA (A2030-N6)- methyltransferase RlmJ in complex with S-adenosylmethionine (AdoMet)

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + adenine(2030) in 23S rRNA = S-adenosyl-L-homocysteine + N(6)-methyladenine(2030) in 23S rRNA
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA large subunit methyltransferase J Chains: A, B
Molecule details ›
Chains: A, B
Length: 289 amino acids
Theoretical weight: 33.09 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37634 (Residues: 1-280; Coverage: 100%)
Gene names: JW3466, b3499, rlmJ, yhiR
Sequence domains: Ribosomal RNA large subunit methyltransferase D, RlmJ
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAM 2 x SAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 46.68Å b: 77.57Å c: 82.7Å
α: 90° β: 103.5° γ: 90°
R-values:
R R work R free
0.172 0.17 0.213
Expression system: Escherichia coli BL21(DE3)