4bkq

X-ray diffraction
2.3Å resolution

Enoyl-ACP reductase from Yersinia pestis (wildtype)with cofactor NADH

Released:

Function and Biology Details

Reaction catalysed:
An acyl-[acyl-carrier protein] + NAD(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enoyl-[acyl-carrier-protein] reductase [NADH] Chain: A
Molecule details ›
Chain: A
Length: 422 amino acids
Theoretical weight: 45.94 KDa
Source organism: Yersinia pestis A1122
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8Z9U1 (Residues: 1-399; Coverage: 100%)
Gene names: YPO4104, YP_4011, fabV, y4119
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAI 1 x NAI
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P6522
Unit cell:
a: 102.628Å b: 102.628Å c: 214.913Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.194 0.23
Expression system: Escherichia coli BL21(DE3)