X-ray diffraction
1.9Å resolution

Enoyl-ACP reducatase FabV from Burkholderia pseudomallei (apo)

Source organism: Burkholderia pseudomallei
Entry authors: Hirschbeck MW, Neckles C, Tonge PJ, Kisker C

Function and Biology Details

Reactions catalysed:
Acyl-CoA + NAD(+) = trans-didehydroacyl-CoA + NADH
An acyl-[acyl-carrier protein] + NAD(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Enoyl-[acyl-carrier-protein] reductase [NADH] Chain: A
Molecule details ›
Chain: A
Length: 417 amino acids
Theoretical weight: 44.97 KDa
Source organism: Burkholderia pseudomallei
Expression system: Escherichia coli BL21(DE3)
  • Canonical: A0A0E1S3N2 (Residues: 1-397; Coverage: 100%)
Gene names: BURPS305_1051, fabV
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 48.64Å b: 93.32Å c: 100.86Å
α: 90° β: 90° γ: 90°
R R work R free
0.176 0.174 0.21
Expression system: Escherichia coli BL21(DE3)