X-ray diffraction
1.52Å resolution

Crystal structure of 3-hydroxybenzoate 6-hydroxylase uncovers lipid- assisted flavoprotein strategy for regioselective aromatic hydroxylation: Platinum derivative


Function and Biology Details

Reaction catalysed:
Salicylate + NADH + O(2) = catechol + NAD(+) + H(2)O + CO(2)
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
FAD_binding_3 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 424 amino acids
Theoretical weight: 46.95 KDa
Source organism: Rhodococcus jostii RHA1
Expression system: Escherichia coli
  • Canonical: Q0SFK6 (Residues: 1-399; Coverage: 100%)
Gene name: RHA1_ro01869
Sequence domains: FAD binding domain
Structure domains:

Ligands and Environments

Cofactor: Ligand FAD 1 x FAD
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: I4122
Unit cell:
a: 106.61Å b: 106.61Å c: 142.31Å
α: 90° β: 90° γ: 90°
R R work R free
0.175 0.174 0.205
Expression system: Escherichia coli