› 4bji

X-ray diffraction
1.45Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126954 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription factor tau subunit sfc1 Chain: A
Molecule details ›
Chain: A
Length: 215 amino acids
Theoretical weight: 25.18 KDa
Source organism: Schizosaccharomyces pombe 927
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O14229 (Residues: 186-396; Coverage: 46%)
Gene names: SPAC6F12.11c, sfc1, tfc1
Sequence domains: RNA polymerase III transcription factor (TF)IIIC subunit HTH domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 35.38Å b: 67.64Å c: 88.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.152 0.17
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

Comparative overview of RNA polymerase II and III transcription cycles, with focus on RNA polymerase III termination and reinitiation.
Arimbasseri et al. (2014)
4 more

2 mentions without citation

RNA polymerase III-specific general transcription factor IIIC contains a heterodimer resembling TFIIF Rap30/Rap74.
Taylor et al. (2013)
1 more