› 4bi8

X-ray diffraction
2Å resolution

Crystal structure of the type VI effector-immunity complex Ssp1-Rap1a from Serratia marcescens

Released:
DOI: 10.2210/pdb4bi8/pdb
PDB entry 4bi8 coloured by chain, front view.
PDB entry 4bi8 coloured by chain, side view.
PDB entry 4bi8 coloured by chain, top view.
Source organism: Serratia marcescens
Primary publication:
Structural basis for type VI secreted peptidoglycan DL-endopeptidase function, specificity and neutralization in Serratia marcescens.
Srikannathasan V, English G, Bui NK, Trunk K, O'Rourke PE, Rao VA, Vollmer W, Coulthurst SJ, Hunter WN
Acta Crystallogr D Biol Crystallogr 69 2468-82 (2013)
PMID: 24311588

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197415 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rap1a immunity protein domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 101 amino acids
Theoretical weight: 11.5 KDa
Source organism: Serratia marcescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: S4S1W3 (Residues: 1-101; Coverage: 100%)
Sequence domains: Rap1a immunity proteins
Structure domains: 10k-s Protein, Hypothetical Protein A; Chain A
SSP1 Chain: B
Molecule details ›
Chain: B
Length: 163 amino acids
Theoretical weight: 18.21 KDa
Source organism: Serratia marcescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: S4S1W1 (Residues: 1-163; Coverage: 100%)
Gene name: AB868_03632
Sequence domains: Type VI secretion system (T6SS), amidase effector protein 4
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P3221
Unit cell:
a: 68.47Å b: 68.47Å c: 92.52Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.175 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Effector⁻Immunity Pairs Provide the T6SS Nanomachine its Offensive and Defensive Capabilities.
Yang et al. (2018)
19 other articles

3 mentions without citation

Molecular weaponry: diverse effectors delivered by the Type VI secretion system.
Alcoforado Diniz et al. (2015)
2 more