4bi6

X-ray diffraction
1.45Å resolution

CRYSTAL STRUCTURE OF A TRIPLE MUTANT (A198V, C202A AND C222N) OF TRIOSEPHOSPHATE ISOMERASE FROM GIARDIA LAMBLIA. COMPLEXED WITH 2- PHOSPHOGLYCOLIC ACID

Released:

Function and Biology Details

Reactions catalysed:
D-glyceraldehyde 3-phosphate = glycerone phosphate
Glycerone phosphate = methylglyoxal + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Triosephosphate isomerase Chain: A
Molecule details ›
Chain: A
Length: 257 amino acids
Theoretical weight: 27.95 KDa
Source organism: Giardia intestinalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P36186 (Residues: 1-257; Coverage: 100%)
Sequence domains: Triosephosphate isomerase
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: I222
Unit cell:
a: 55.578Å b: 100.474Å c: 118.147Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.19 0.207
Expression system: Escherichia coli BL21(DE3)