4bhu

X-ray diffraction
1.91Å resolution

Crystal structure of BslA - A bacterial hydrophobin

Released:
Entry authors: Rao FV, Hobley L, Ostrowski A, Bromley KM, Porter M, Prescott AR, Swedlow JR, MacPhee CE, van Aalten DMF, Stanley-Wall NR

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Biofilm-surface layer protein A Chains: A, B, C, D, E, F, G, H, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, J
Length: 130 amino acids
Theoretical weight: 14.11 KDa
Source organism: Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10
Expression system: Escherichia coli B
UniProt:
  • Canonical: P71014 (Residues: 48-172; Coverage: 82%)
Gene names: BSU31080, bslA, yuaB
Sequence domains: Biofilm surface layer A
Structure domains: Immunoglobulin-like
Biofilm-surface layer protein A Chain: I
Molecule details ›
Chain: I
Length: 130 amino acids
Theoretical weight: 14.11 KDa
Source organism: Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10
Expression system: Escherichia coli B
UniProt:
  • Canonical: P71014 (Residues: 48-172; Coverage: 82%)
Gene names: BSU31080, bslA, yuaB
Sequence domains: Biofilm surface layer A
Structure domains: Immunoglobulin-like

Ligands and Environments

2 bound ligands:
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 69.7Å b: 95.98Å c: 259.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.165 0.199
Expression system: Escherichia coli B