4b98

X-ray diffraction
1.65Å resolution

The structure of the omega aminotransferase from Pseudomonas aeruginosa

Released:
Source organism: Pseudomonas aeruginosa
Entry authors: Sayer C, Isupov MN, Westlake A, Littlechild JA

Function and Biology Details

Reaction catalysed:
L-alanine + 3-oxopropanoate = pyruvate + beta-alanine
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-alanine--pyruvate aminotransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 448 amino acids
Theoretical weight: 48.43 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9I700 (Residues: 1-448; Coverage: 100%)
Gene names: PA0132, bauA
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 4 x PLP
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 119.23Å b: 192.47Å c: 77.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.22 0.26
Expression system: Escherichia coli BL21