› 4b8y

X-ray diffraction
1.9Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164307 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fe/B12 periplasmic-binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 277 amino acids
Theoretical weight: 31.38 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2FVW9 (Residues: 27-302; Coverage: 98%)
Gene name: SAOUHSC_02554
Sequence domains: Periplasmic binding protein
Structure domains: Nitrogenase molybdenum iron protein domain
VIRULENCE FACTOR Chain: B
Molecule details ›
Chain: B
Length: 6 amino acids
Theoretical weight: 706 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand EDO 10 x EDO
Ligand CL 1 x CL
Ligand FE 1 x FE
1 modified residue:
Ligand AHO 3 x AHO

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P21
Unit cell:
a: 53.89Å b: 50.14Å c: 54.35Å
α: 90° β: 106.44° γ: 90°
R-values:
R R work R free
0.176 0.174 0.223
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

7 review citations

Recent developments in understanding the iron acquisition strategies of gram positive pathogens.
Sheldon et al. (2015)
6 more

2 mentions without citation

Iron Metabolism at the Interface between Host and Pathogen: From Nutritional Immunity to Antibacterial Development.
Marchetti et al. (2020)
1 more