4b8w

X-ray diffraction
2.75Å resolution

Crystal structure of human GDP-L-fucose synthase with bound NADP and GDP, tetragonal crystal form

Released:
Source organism: Homo sapiens
Entry authors: Vollmar M, Krojer T, Shafqat N, Rojkova A, Bunkoczi G, Pike A, Chaikuat A, Carpenter E, Yue WW, Kavanagh K, von Delft F, Weigelt J, Arrowsmith CH, Bountra C, Edwards A, Oppermann U

Function and Biology Details

Reaction catalysed:
GDP-beta-L-fucose + NADP(+) = GDP-4-dehydro-alpha-D-rhamnose + NADPH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GDP-L-fucose synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 319 amino acids
Theoretical weight: 35.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13630 (Residues: 7-320; Coverage: 98%)
Gene names: GFUS, SDR4E1, TSTA3
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 2 x NAP
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P42
Unit cell:
a: 111.47Å b: 111.47Å c: 46.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.201 0.252
Expression system: Escherichia coli BL21(DE3)