4b7y

X-ray diffraction
3.25Å resolution

Crystal structure of the MSL1-MSL2 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Male-specific lethal 1 homolog Chains: A, B
Molecule details ›
Chains: A, B
Length: 44 amino acids
Theoretical weight: 4.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q68DK7 (Residues: 212-252; Coverage: 7%)
Gene names: MSL1, MSL1L1
Sequence domains: Dimerisation domain of Male-specific-Lethal 1
E3 ubiquitin-protein ligase MSL2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 116 amino acids
Theoretical weight: 13.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HCI7 (Residues: 1-116; Coverage: 20%)
Gene names: KIAA1585, MSL2, MSL2L1, RNF184
Sequence domains: zinc RING finger of MSL2
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3221
Unit cell:
a: 100.99Å b: 100.99Å c: 88.65Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.245 0.244 0.26
Expression system: Escherichia coli BL21(DE3)