X-ray diffraction
1.92Å resolution

Crystal structures of divalent metal dependent pyruvate aldolase, HpaI, in complex with ketobutyrate


Function and Biology Details

Reaction catalysed:
4-hydroxy-2-oxoheptanedioate = pyruvate + succinate semialdehyde
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
4-hydroxy-2-oxo-heptane-1,7-dioate aldolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 251 amino acids
Theoretical weight: 27.01 KDa
Source organism: Escherichia coli ATCC 8739
Expression system: Escherichia coli BL21(DE3)
  • Canonical: B1IS70 (Residues: 1-251; Coverage: 96%)
Gene names: EcolC_3707, hpaI, hpcH
Sequence domains: HpcH/HpaI aldolase/citrate lyase family
Structure domains: Phosphoenolpyruvate-binding domains

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P213
Unit cell:
a: 151.327Å b: 151.327Å c: 151.327Å
α: 90° β: 90° γ: 90°
R R work R free
0.158 0.155 0.186
Expression system: Escherichia coli BL21(DE3)