4b3j

X-ray diffraction
2.5Å resolution

Crystal structure of Mycobacterium tuberculosis fatty acid beta- oxidation complex with CoenzymeA bound at the hydratase and thiolase active sites

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
3-hydroxyacyl-CoA dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 736 amino acids
Theoretical weight: 78.01 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O53872 (Residues: 1-720; Coverage: 100%)
Gene names: Rv0860, fadB
Sequence domains:
Structure domains:
Putative acyltransferase Rv0859 Chains: C, D
Molecule details ›
Chains: C, D
Length: 403 amino acids
Theoretical weight: 42.46 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O53871 (Residues: 1-403; Coverage: 100%)
Gene names: Rv0859, fadA
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2
Unit cell:
a: 248.3Å b: 135.25Å c: 118.58Å
α: 90° β: 110.64° γ: 90°
R-values:
R R work R free
0.183 0.181 0.221
Expression system: Escherichia coli BL21