4b3g

X-ray diffraction
2.85Å resolution

crystal structure of Ighmbp2 helicase in complex with RNA

Released:

Function and Biology Details

Reaction catalysed:
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
DNA-binding protein SMUBP-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 646 amino acids
Theoretical weight: 71.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P38935 (Residues: 3-648; Coverage: 65%)
Gene names: IGHMBP2, SMBP2, SMUBP2
Sequence domains:
Structure domains:
RNA (5'-(AP*AP*AP*AP*AP*AP*AP*AP*AP)-3') Chains: G, H
Molecule details ›
Chains: G, H
Length: 9 nucleotides
Theoretical weight: 2.92 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF
Spacegroup: P3121
Unit cell:
a: 87.288Å b: 87.288Å c: 372.692Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.206 0.292
Expression systems:
  • Escherichia coli BL21
  • Not provided