X-ray diffraction
1.59Å resolution

Humanised monomeric RadA in complex with 5-methyl indole

Source organism: Pyrococcus furiosus
Entry authors: Scott DE, Ehebauer MT, Pukala T, Marsh M, Blundell TL, Venkitaraman AR, Abell C, Hyvonen M

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
DNA repair and recombination protein RadA Chains: A, C
Molecule details ›
Chains: A, C
Length: 231 amino acids
Theoretical weight: 25.5 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli BL21(DE3)
  • Canonical: O74036 (Residues: 108-349; Coverage: 66%)
Gene names: PF1926, radA
Sequence domains: Rad51
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 40.28Å b: 87.246Å c: 62.628Å
α: 90° β: 91.5° γ: 90°
R R work R free
0.18 0.179 0.212
Expression system: Escherichia coli BL21(DE3)