4b30

X-ray diffraction
2.1Å resolution

Structure of the mutant V44A of the fluorescent protein KillerRed

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174038 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
KillerRed Chain: A
Molecule details ›
Chain: A
Length: 235 amino acids
Theoretical weight: 26.42 KDa
Source organism: Anthomedusae sp. DC-2005
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2TCH5 (Residues: 1-237; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein
KillerRed Chain: B
Molecule details ›
Chain: B
Length: 235 amino acids
Theoretical weight: 26.4 KDa
Source organism: Anthomedusae sp. DC-2005
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2TCH5 (Residues: 1-237; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P62
Unit cell:
a: 134.31Å b: 134.31Å c: 76.22Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.167 0.165 0.199
Expression system: Escherichia coli BL21(DE3)