4b2x

X-ray diffraction
1.7Å resolution

Pseudomonas aeruginosa RmlA in complex with allosteric inhibitor

Released:
Source organism: Pseudomonas aeruginosa PAO1
Entry authors: Alphey MS, Pirrie L, Torrie LS, Gardiner M, Sarkar A, Brenk R, Westwood NJ, Gray D, Naismith JH

Function and Biology Details

Reaction catalysed:
dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-alpha-D-glucose
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-1-phosphate thymidylyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 303 amino acids
Theoretical weight: 33.66 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9HU22 (Residues: 1-293; Coverage: 100%)
Gene names: PA5163, rmlA
Sequence domains: Nucleotidyl transferase
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 64.23Å b: 154.83Å c: 134.49Å
α: 90° β: 92.19° γ: 90°
R-values:
R R work R free
0.22 0.218 0.253
Expression system: Escherichia coli BL21