› 4b2i

X-ray diffraction
1.3Å resolution

Humanised monomeric RadA in complex with indazole

Released:
DOI: 10.2210/pdb4b2i/pdb
PDB 4b2i coloured by chain and viewed from the front.
PDB 4b2i coloured by chain and viewed from the side.
PDB 4b2i coloured by chain and viewed from the top.
Source organism: Pyrococcus furiosus
Primary publication:
Using a Fragment-Based Approach To Target Protein-Protein Interactions.
Scott DE, Ehebauer MT, Pukala T, Marsh M, Blundell TL, Venkitaraman AR, Abell C, Hyvönen M
Chembiochem (2013)
PMID: 23344974

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA repair and recombination protein RadA Chain: A
Molecule details ›
Chain: A
Length: 231 amino acids
Theoretical weight: 25.5 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O74036 (Residues: 108-349; Coverage: 66%)
Gene names: PF1926, radA
Sequence domains: Rad51
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand LZ1 1 x LZ1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 37.62Å b: 78.77Å c: 39.63Å
α: 90° β: 118.06° γ: 90°
R-values:
R R work R free
0.2 0.199 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Druggable binding sites in the multicomponent assemblies that characterise DNA double-strand-break repair through non-homologous end joining.
Kefala Stavridi et al. (2020)
15 more

2 mentions without citation

Using a fragment-based approach to target protein-protein interactions.
Scott et al. (2013)
1 more