4b2c

X-ray diffraction
1.43Å resolution

Structure of the factor Xa-like trypsin variant triple-Ala (TPA) in complex with eglin C

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cationic trypsin Chains: A, C
Molecule details ›
Chains: A, C
Length: 223 amino acids
Theoretical weight: 23.39 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00760 (Residues: 24-246; Coverage: 97%)
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Eglin C Chains: B, D
Molecule details ›
Chains: B, D
Length: 71 amino acids
Theoretical weight: 8.17 KDa
Source organism: Hirudo medicinalis
Expression system: Escherichia coli
UniProt:
  • Canonical: P01051 (Residues: 1-70; Coverage: 100%)
Sequence domains: Potato inhibitor I family
Structure domains: Trypsin Inhibitor V, subunit A

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X12
Spacegroup: P21
Unit cell:
a: 79.21Å b: 37.02Å c: 103.96Å
α: 90° β: 102.26° γ: 90°
R-values:
R R work R free
0.158 0.158 0.182
Expression system: Escherichia coli