X-ray diffraction
1.36Å resolution

Structure of the factor Xa-like trypsin variant triple-Ala (TGPA) in complex with eglin C


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cationic trypsin Chains: A, C
Molecule details ›
Chains: A, C
Length: 223 amino acids
Theoretical weight: 23.44 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
  • Canonical: P00760 (Residues: 24-246; Coverage: 97%)
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Eglin C Chains: B, D
Molecule details ›
Chains: B, D
Length: 71 amino acids
Theoretical weight: 8.17 KDa
Source organism: Hirudo medicinalis
Expression system: Escherichia coli
  • Canonical: P01051 (Residues: 1-70; Coverage: 100%)
Sequence domains: Potato inhibitor I family
Structure domains: Trypsin Inhibitor V, subunit A

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 78.34Å b: 36.94Å c: 103.02Å
α: 90° β: 103.41° γ: 90°
R R work R free
0.184 0.183 0.204
Expression system: Escherichia coli