X-ray diffraction
1.89Å resolution

Structure of the factor Xa-like trypsin variant triple-Ala (TGA) in complex with eglin C


Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cationic trypsin Chains: A, C
Molecule details ›
Chains: A, C
Length: 223 amino acids
Theoretical weight: 23.39 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
  • Canonical: P00760 (Residues: 24-246; Coverage: 97%)
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Eglin C Chains: B, D
Molecule details ›
Chains: B, D
Length: 66 amino acids
Theoretical weight: 7.68 KDa
Source organism: Hirudo medicinalis
Expression system: Escherichia coli
  • Canonical: P01051 (Residues: 5-70; Coverage: 94%)
Sequence domains: Potato inhibitor I family
Structure domains: Trypsin Inhibitor V, subunit A

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 78.8Å b: 35.11Å c: 105.39Å
α: 90° β: 104.26° γ: 90°
R R work R free
0.204 0.203 0.259
Expression system: Escherichia coli