4b0b

X-ray diffraction
1.9Å resolution

Crystal Structure of 3-hydroxydecanoyl-Acyl Carrier Protein Dehydratase (FabA) from Pseudomonas aeruginosa in complex with 3-(pyridin-2-yloxy)aniline

Released:

Function and Biology Details

Reactions catalysed:
A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H(2)O
Trans-dec-2-enoyl-[acyl-carrier-protein] = cis-dec-3-enoyl-[acyl-carrier-protein]
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 171 amino acids
Theoretical weight: 18.77 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O33877 (Residues: 1-171; Coverage: 100%)
Gene names: PA1610, fabA
Sequence domains: FabA-like domain
Structure domains: Hotdog Thioesterase

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX
Spacegroup: P21212
Unit cell:
a: 59.32Å b: 117.99Å c: 47.98Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.203 0.236
Expression system: Escherichia coli BL21(DE3)