› 4azj

X-ray diffraction
1.5Å resolution

Structural basis of L-phosphoserine binding to Bacillus alcalophilus phosphoserine aminotransferase

Released:
DOI: 10.2210/pdb4azj/pdb
PDB entry 4azj coloured by chain, front view.
PDB entry 4azj coloured by chain, side view.
PDB entry 4azj coloured by chain, top view.
Source organism: Alkalihalobacillus alcalophilus
Primary publication:
Structural basis of L-phosphoserine binding to Bacillus alcalophilus phosphoserine aminotransferase.
Battula P, Dubnovitsky AP, Papageorgiou AC
Acta Crystallogr D Biol Crystallogr 69 804-11 (2013)
PMID: 23633589

Function and Biology Details

Reaction catalysed: 
4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193203 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoserine aminotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 360 amino acids
Theoretical weight: 40.24 KDa
Source organism: Alkalihalobacillus alcalophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RME2 (Residues: 2-361; Coverage: 100%)
Gene name: serC
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
3 bound ligands:
Ligand SEP 2 x SEP
Ligand CL 2 x CL
Ligand NA 3 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: C2221
Unit cell:
a: 104.692Å b: 136.194Å c: 151.283Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.146 0.165
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

PSAT1 Regulated Oxidation-Reduction Balance Affects the Growth and Prognosis of Epithelial Ovarian Cancer.
Zhang et al. (2020)
3 more

1 mention without citation

Structural Analysis of Phosphoserine Aminotransferase (Isoform 1) From Arabidopsis thaliana- the Enzyme Involved in the Phosphorylated Pathway of Serine Biosynthesis.
Sekula et al. (2018)