› 4azg

X-ray diffraction
2.4Å resolution

Differential inhibition of the tandem GH20 catalytic modules in the pneumococcal exo-beta-D-N-acetylglucosaminidase, StrH

Released:
DOI: 10.2210/pdb4azg/pdb
PDB entry 4azg coloured by chain, front view.
PDB entry 4azg coloured by chain, side view.
PDB entry 4azg coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Primary publication:
Inhibition of the family 20 glycoside hydrolase catalytic modules in the Streptococcus pneumoniae exo-β-D-N-acetylglucosaminidase, StrH.
Pluvinage B, Stubbs KA, Hattie M, Vocadlo DJ, Boraston AB
Org Biomol Chem 11 7907-15 (2013)
PMID: 24132305

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155969 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-N-acetylhexosaminidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 443 amino acids
Theoretical weight: 49.79 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli
UniProt:
  • Canonical: P49610 (Residues: 627-1064; Coverage: 34%)
Gene names: SP_0057, strH
Sequence domains: Glycosyl hydrolase family 20, catalytic domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand OAN 2 x OAN
Ligand ACT 6 x ACT
Ligand EDO 9 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 66.7Å b: 115.2Å c: 129.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.19 0.248
Expression system: Escherichia coli

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Citations

1 review citation

Glycan-metabolizing enzymes in microbe-host interactions: the Streptococcus pneumoniae paradigm.
Hobbs et al. (2018)
5 other articles