4ay5

X-ray diffraction
3.15Å resolution

Human O-GlcNAc transferase (OGT) in complex with UDP and glycopeptide

Released:

Function and Biology Details

Reaction catalysed:
UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-serine = UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 723 amino acids
Theoretical weight: 80.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O15294 (Residues: 323-1041; Coverage: 69%)
Gene name: OGT
Sequence domains:
Structure domains:
TGF-beta-activated kinase 1 and MAP3K7-binding protein 1 Chains: I, J, K, L
Molecule details ›
Chains: I, J, K, L
Length: 11 amino acids
Theoretical weight: 1.12 KDa
Source organism: synthetic construct
Expression system: Not provided
UniProt:
  • Canonical: Q15750 (Residues: 389-399; Coverage: 2%)
Gene names: MAP3K7IP1, TAB1

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P321
Unit cell:
a: 274.17Å b: 274.17Å c: 142.15Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.174 0.204
Expression systems:
  • Escherichia coli BL21
  • Not provided